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KMID : 0376219800170020491
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Chonnam Medical Journal
1980 Volume.17 No. 2 p.491 ~ p.498
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Control of the Uptake of Methylene Blue in Erythrocytes
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Abstract
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The uptake of methylene blue by rabbit erythrocytes in the presence of glucose was enhanced when the unionized moiety of methylene blue was increased either by the addition of ionic compounds in the medium or by changing the pH of the medium to the alkaline side. The enhancing effect of ionic compounds was markedly diminished by pre-treatment of erythrocytes with pancreatic lipase, but the methylene blue uptake was not influenced by ouabain. On the other hand, methylene blue uptake was markedly inhibited by the addition of inorganic phosphate in the medium which is known to reduce the glucose-6-phosphate level in erythrocytes.
These results indicate that the membrane transport of methylene blue in erythrocytes occurs by simple passive diffusion of the unionized moiety of the basic dye across a red cell membrane which is preferentially permeable to this form and impermeable to the ionized form. It is also suggested that the uptake of methylene blue by erythrocytes can be controlled independently by modulating the red cell glycolysis.
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